Coding

Part:BBa_K4998029:Design

Designed by: Rafail Andreou   Group: iGEM23_thessaloniki   (2023-10-09)


bsrG toxin gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For our project, Euphoresis, we used a B. subtilis strain, WB800N. The bsrG is a B. subtilis toxin, that is why we inserted the mRNA sequence as a whole. In this way the toxin's mRNA will fold appropriately in the B. subtilis strains.


Source

Bacillus subtilis

References